The present manuscript describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic method for the simultaneous determination of Nifedipine (NFP) and Lidocaine (LDC) in cream. Derivative spectroscopy offers a useful approach for the analysis of drugs in cream. In this study a first-derivative spectroscopic method was used for simultaneous determination of Nifedipine and Lidocaine using the zero-crossing technique. The measurements were carried out at wavelengths of 257 nm and 235 nm for Nifedipine and Lidocaine respectively. The method was found to be linear (r2=0.991) in the range of 2-28 μg/ml for Nifedipine at 257 nm. The linear correlation was obtained (r2=0.990) in the range of 2-28 μg/ml for Lidocaine at 235 nm. The limit of detection was found to be 0.39 and 0.77 μg/ml for Nifedipine and Lidocaine respectively. The limit of quantification was found to be 1.19 and 2.34 μg/ml respectively. The method was successfully applied for simultaneous determination of Nifedipine and Lidocaine in cream.
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